Cytotoxicity of zinc nanoparticles biosynthesized by microorganisms on human keratinocyte cell line

LOPEZ VENDITTI, ELIANA; CALIENI, NATALIA; MONTANARI, JORGE; BUSTOS, PAMELA SOLEDAD; PAEZ, PAULINA; GUIÑAZU ALANIZ, NATALIA LORENA

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigacion Clínica (SAIC) - Reunión Anual de Sociedades de Biociencias 2019; 2019

Sociedad Argentina de Investigacion Clinica

Metal nanoparticles ?NPs? (10-100 nm) have an important biocidal activity with possible biomedical applications. Zinc NPs (ZnNPs) are widely used for cosmetical porpoises. . The aim of this work was to investigate the toxicity of Zinc NPs (ZnNPs) biosynthesized by microorganisms, in a human keratinocyte cell line -HaCaT.ZnNPs were synthesized using Pseudomonas aeruginosa (ATCC strain) and were characterized by UV-Vis spectroscopy and by transmission electron microscopy. HaCaT cells were incubated for 4 and 24 h at different ZnNPs dilutions of (1/2,1/5,1/10). RPMI 1640 5% SBF culture medium, a metal precursor salt solution ZnSO4 (0.1 and 0.25 mM), and a bacterial growth control of biosynthesis (CCB) were used as controls. Cell viability was evaluated by MTT, crystal violet and neutral red tests; reactive oxygen species (ROS) were studied by DCF-DA, superoxide dismutase (SOD) activity was determined by the riboflavin-NBT method; and glutathione (GSH) content by the Ellman method. NPs Cell capture assay was performed by fluorescence microscopy and changes in cell migration were evaluated by the wound healing assay.As determined by fluorescence microscopy ZnNP were able to enter HaCat cells. All toxicity assays indicated that cell viability was significantly altered by ZnNP 1/2 and CCB conditions after 4 and 24 h incubation. ROS levels increased after 4 h incubation with ZnNP 1/2, 1/5, and CCB, while a 24 h incubation 1/10 dilution also augmented ROS. SOD activity increases at all ZnNP dilutions tested and with CCB. GSH content was not modified by any treatment. Finally, the presence of ZnNP and CCB in the culture media affected cell migration.Altogether these results suggest that ZnNPs are able not only to enter into skin cells but also to modify human keratinocyte viability, oxidant/antioxidant cell balance and cell migration. More studies are needed to unravel the mechanisms underlying these alterations.