Mechanism of Neonicotinoid Toxicity in Human Trophoblasts

GOMEZ DIEGO SEBASTIAN; BUSTOS PAMELA; GUIÑAZÚ NATALIA

Mar del Plata

Congreso; LXIII Reunión conjunta SAIC SAI SAFIS; 2018

Sociedades SAIC SAI SAFIS

Neonicotinoid (NEO) insecticides are a relatively new class of pesticides developed to replace other insecticides. NEO were thought to have limited toxicity to mammals, since their higher specificity toward insects. Nevertheless NEO toxicity in non-target organisms is not widely studied. We have demonstrated that the NEO acetamiprid (Ace) and its commercial formulation (CFace) are cytotoxic to human trophoblast cell lines, and trigger reactive oxygen species production. Moreover, CFace is more toxic to trophoplasts than Ace. The aim of this study was to investigate the toxicity mechanisms of Ace and CFace in a human trophoblast cell line.Human trophoblst HTR-8/SVneo cells were exposed to Ace or CFace (0.1-100 µM) for 4 and 24 h. Antioxidant enzyme activities (catalase, superoxide dismutase and glutathione s-transferase), as well as protein oxidation ?PO? (advanced oxidation protein products method) and genotoxic damage ?GD? (comet alkaline assay) were determined under these conditions.Exposure to Ace and CFace modulated antioxidant enzymes: Ace reduced activities at high concentrations (10-100 µM) at 24 h (p