CONICET | Buscador de Institutos y Recursos Humanos

Differences in the susceptibility degree to Trypanosoma cruzi infection have been reported. BALB/c mice are susceptible to infection and autoimmunity, whereas C57BL/6 mice are resistant. Previously we demonstrated that BALB/c mice immunized with cruzipain (Cz) showed an increase in spleen B lymphocytes (LB), it was related with the presence of autoantibodies to cardiac myosin. In C57BL/6 mice immunized with Cz these alterations were absent. The purpose of this work was to study B cell activation markers such as a) CD23 and b) MHC-II. Spleen cells (SC) were obtained at day 14 after the third immunization, from BALB/c and C57BL/6 mice immunized with: Cz + CFA (immune group, n=4) and OVA + CFA (control group, n=4). Each group received three i.d. injections containing 10 mg of Cz or OVA. a) CD23 expression was analyzed in SC obtained from both mouse strains and cultivated for 24 h. b) MHC-II expression was analyzed in purified LB, obtained from BALB/c mice and incubated with LPS or medium for 24 h. LB purification was done using Dynabeads mouse pan T (Thy1.2) and plastic adherence. Results: We demonstrate that in BALB/c mice, Cz immunization increased the number of SC with CD23+ marker in BALB/c mice compared with C57BL/6 SC (23 ± 4% vs 8 ± 4 %). Purified LB from BALB/c immune mice when were stimulated with LPS or medium alone showed an increased expression of MHC-II. Mean values for fluorescence intensity ± SD were, immune vs control, 776 ± 70 vs 530 ± 6 (medium), 795 ± 50 vs 596 ± 30 (LPS). Conclusion: BALB/c, a mouse strain susceptible to infection and autoimmunity when is immunized with Cz, shows a LB higher activation compared with control ones. In addition, these cells show a higher activation state compared with the ones derived from C57BL/6, a mouse strain resistant to infection and autoimmunity

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