A linkage map for hazelnut (Corylus avellana L.)

MEHLENBACHER S., BROWN R, BASSIL N, ; NOUHRA E; T. KUBISIAK

ACTA HORTICULTURAE

International Society for Horticultural Science

Año: 2005 vol. 686 p. 135 - 140

0567-7572

A linkage map for European hazelnut (Corylus avellana L.) was constructed using random amplified polymorphic DNA (RAPD) markers and a seedling population from the cross OSU 252.146 x OSU 414.062. A total of 1420 decamer primers were screened using template DNA of six seedlings and the two parents. Primers that generated easily-scored polymorphic bands were identified and used to amplify DNA of all seedlings in the population. A total of 730 markers were scored: 572 markers that segregated 1:1 and 158 markers that segregated 3:1. Maps were constructed using JoinMap 3.0, initially for each chromosome in each parent. The corresponding chromosome in the susceptible parent was then identified for each chromosome in the resistant parent based on grouping with common markers that segregated 3:1. Merging of the three data sets was possible for nine groups. The nine resulting linkage groups spanned 601 cM and included 151 markers (1:1) from the resistant parent, 146 markers (1:1) from the susceptible parent, and 88 markers segregating 3:1. Two additional groups consisting of 51 markers and spanning 158 cM were identified, but JoinMap was not able to merge the data sets. We assume that these large groups correspond to the 11 pairs of chromosomes in C. avellana. Additional loci were assigned to these 11 groups but could not be placed on the map. Several additional small linkage groups were also identified.