DETERMINACIÓN DE LA EFICIENCIA DE LA MICROPROPAGACIÓN DE GENOTIPOS DE MANDIOCA (MANIHOT ESCULENTA, EUPHORBIACEAE) DE INTERÉS PÁRA EL NORDESTE ARGENTINO.

MEDINA, RICARDO D.; SCHALLER, SILVIA; DOLCE, NATALIA R.; MROGINSKI, LUIS A.

BOLETíN DE LA SOCIEDAD ARGENTINA DE BOTáNICA

SOCIEDAD ARGENTINA DE BOTÁNICA

Lugar: Córdoba; Año: 2017 vol. 52 p. 497 - 505

0373-580X

In vitro cassava propagation has been reported in numerous studies; however, the efficiency of multiplication had only been established in a very restricted number of cultivars and/or subcultures. In order to evaluate the in vitro performance of 20 economically important cassava cultivars of Northeast Argentina during the multiplication phase (6 total subcultures performed every 30 days), uninodal segments were cultured in basal Murashige and Skoog (1962) supplemented with 0.01 mg/L of 1-naphthaleneacetic acid, 0.01 mg/L of 6-benzylaminopurine and 0.1 mg/L gibberellic acid. Plant regeneration was possible in 100% of the cultivars during the 6 subcultures. Regardless of the subculture, the average number of nodes regenerated per explant varied significantly by cultivar (P0,0001), between 2 to 5 nodes. The average number of accumulated nodes was significantly different according to the cultivar, achieving values between 46 to 16,568 nodes. Three clusters of cultivars were differentiated, in relation to its propagation and growth in length ability. Knowledge of the multiplication rate of different cultivars will allow adjusting protocols to optimize the efficiency of cassava micropropagation.