INVESTIGADORES
MARTINEZ PASTUR Guillermo Jose
artículos
Título:
Role of polyamines during in vitro rhizogenesis of Nothofagus nervosa using successive culture media.
Autor/es:
MARTÍNEZ PASTUR G; ARENA, ME; MP BENAVIDES; E ELIASCO; N CURVETTO
Revista:
NEW FORESTS
Editorial:
Elsevier
Referencias:
Año: 2007 vol. 34 p. 83 - 93
ISSN:
0169-4286
Resumen:
An in vitro rooting protocol for producing Nothofagus nervosa microplants
using successive steps during tissue culture is reported. Qualitative and
quantitative changes in putrescine, spermidine, and spermine tissue contents, during
the rooting process and their correlation with other biochemical markers and morphological
changes, provided the rationale for their use during the study of a successive
in vitro culture protocol for N. nervosa, as well as the use of their inhibitors.
The polyamine tissue content was maximum before root emergence (spermine
raised to 100 nmol/g FW to later decreased to ca. 50 nmol/g FW in the following
days), but it was lower in improved rooting media (197 nmol/g FW compared to
264 nmol/g FW of the control medium). It was concluded that polyamines qualitatively
and quantitatively improve in vitro rhizogenesis, and the best successive culture
media included 10 lM spermine during the expression phase.Nothofagus nervosa microplants
using successive steps during tissue culture is reported. Qualitative and
quantitative changes in putrescine, spermidine, and spermine tissue contents, during
the rooting process and their correlation with other biochemical markers and morphological
changes, provided the rationale for their use during the study of a successive
in vitro culture protocol for N. nervosa, as well as the use of their inhibitors.
The polyamine tissue content was maximum before root emergence (spermine
raised to 100 nmol/g FW to later decreased to ca. 50 nmol/g FW in the following
days), but it was lower in improved rooting media (197 nmol/g FW compared to
264 nmol/g FW of the control medium). It was concluded that polyamines qualitatively
and quantitatively improve in vitro rhizogenesis, and the best successive culture
media included 10 lM spermine during the expression phase.N. nervosa, as well as the use of their inhibitors.
The polyamine tissue content was maximum before root emergence (spermine
raised to 100 nmol/g FW to later decreased to ca. 50 nmol/g FW in the following
days), but it was lower in improved rooting media (197 nmol/g FW compared to
264 nmol/g FW of the control medium). It was concluded that polyamines qualitatively
and quantitatively improve in vitro rhizogenesis, and the best successive culture
media included 10 lM spermine during the expression phase.lM spermine during the expression phase.