Behaviour of the anti-oxidant defense system and heme oxygenase-1 protein expresión in fructosa-hypertensive rats.

ARIEL POLIZIO; SOLEDAD GONSALES; MARINA C MUÑOZ; CLARA PEÑA; MARÍA L TOMARO

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY

Wiley-Blackwell

Año: 2006 vol. 33 p. 734 - 739

0305-1870

1. Addition of fructose to a rat diet for various periods of time leads to hypertension, hyperinsulinaemia and dyslipidaemia and provides a model for testing oxidative stress parameters in the animals. 2. In the present study, oxidative stress generation, the soluble and enzymatic defence system and heme oxygenase-1 (HO-1) protein expression were investigated in the heart, liver and kidney of rats fed fructose for a period of 1 or 8 months. 3. Compared with the control group, fructose-hypertensive rats showed increased in lipid peroxidation only in the heart after both 1 and 8 months of fructose treatment. Changes in the behaviour of the soluble and enzymatic defence system and HO-1 protein expression were different depending on the organ. Increased or unaltered activities of anti-oxidant enzymes were found in the liver and kidney, respectively. Induction of HO-1 prevented the generation of oxidative stress in the liver, where the activity of anti-oxidant defence enzymes was not reduced. Increased expression of HO-1 protein was not able to prevent the generation of oxidative stress in the heart, where fructose treatment diminished the activity of anti-oxidant enzymes. 4. The results of the present study demonstrate that upregulation of HO-1 may prevent the generation of oxidative stress only when the anti-oxidant defence system is still operative. Key words: anti-oxidant enzymes, fructose hypertension, heme oxygenase, oxidative stress, rats.