Effects of anthracene and magnetite nanoparticles coated with oleic acid on breast cancer cell proliferation

MARDIROSIAN, MARIANA NOELIA; LASAGNA M; HIELPOS MS; NÚÑEZ, M; COCCA, C; VENTURINO A

Congreso; Reunión Anual de Sociedades de Biociencia SAIC ? SAI ? SAFIS 2020; 2020

Sociedad Argentina de Investigación Clínica

The demand for crude oil hydrocarbons represents a growing concernworldwide due to pollution problems from drilling, productionand transportation. Thus, it is important to perform toxicity studies toassess the risk associated with these incidents, not only for the environmentbut also for humans. Our aim was to evaluate the effectsof anthracene, a polycyclic aromatic hydrocarbon (PAH), and magnetitenanoparticles coated with oleic acid (NP) on breast cancercells proliferation. These NP were developed in the CITAAC for theremediation of water contaminated with PAH.We have previously studied the effects of the water-soluble fractionof petroleum (WSF) simulating a possible oil spill. WSF exposuresignificantly decreased the viability and significantly increased catalase (CAT) activity of MCF-7 and MDA-MB-231 breast cancer cellscompared to controls. As the WSF is a mixture of hydrocarbons weconsidered important to study the effects of PAHs individually tounderstand if the observed effects were induced by the individualaction of its components or by the interaction between them.MCF-7 and MDA-MB-231 cell lines were treated for 7d with eitheranthracene (0; 3,5; 7; 15 and 28 μM) or NP (0; 12,5; 25; 50; 100 and200 mg/L) to perform clonogenic assays. No significant differenceswere observed in the clonogenicity of anthracene treated cells comparedto control. However, the exposure to NP from 12,5 to 200 mg/Land from 25 to 200 mg/L significantly decreased the clonogenicityof MCF-7 and MDA-MB-231 cells respectively (p