INVESTIGADORES
SEIJO Jose Guillermo
artículos
Título:
Gene expression analysis at the onset of aposporous apomixis in Paspalum notatum. Plant Molecular Bioloy
Autor/es:
LASPINA, N.V.; T. VEGA; J.G. SEIJO; A.M. GONZÁLEZ; L.G. MARTELOTTO; J. STEIN; M. PODIO; J.P.A. ORTIZ; V.C. ECHENIQUE; C.L. QUARÍN; S.C. PESSINO
Revista:
PLANT MOLECULAR BIOLOGY
Editorial:
Springer
Referencias:
Año: 2008 vol. 67 p. 615 - 628
ISSN:
0167-4412
Resumen:
Abstract Apomixis is a route of asexual reproduction
through seeds, that progresses in the absence of meiosis
and fertilization to generate maternal clonal progenies.
Gametophytic apomicts are usually polyploid and probably
arose from sexual ancestors through a limited number of
mutations in the female reproductive pathway. A differential
display analysis was carried out on immature
inflorescences of sexual and apomictic tetraploid genotypes
of Paspalum notatum, in order to identify genes associated
with the emergence of apospory. Analysis of *10,000
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
with the emergence of apospory. Analysis of *10,000
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
through seeds, that progresses in the absence of meiosis
and fertilization to generate maternal clonal progenies.
Gametophytic apomicts are usually polyploid and probably
arose from sexual ancestors through a limited number of
mutations in the female reproductive pathway. A differential
display analysis was carried out on immature
inflorescences of sexual and apomictic tetraploid genotypes
of Paspalum notatum, in order to identify genes associated
with the emergence of apospory. Analysis of *10,000
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
with the emergence of apospory. Analysis of *10,000
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
Apomixis is a route of asexual reproduction
through seeds, that progresses in the absence of meiosis
and fertilization to generate maternal clonal progenies.
Gametophytic apomicts are usually polyploid and probably
arose from sexual ancestors through a limited number of
mutations in the female reproductive pathway. A differential
display analysis was carried out on immature
inflorescences of sexual and apomictic tetraploid genotypes
of Paspalum notatum, in order to identify genes associated
with the emergence of apospory. Analysis of *10,000
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
with the emergence of apospory. Analysis of *10,000
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
Paspalum notatum, in order to identify genes associated
with the emergence of apospory. Analysis of *10,000
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
*10,000
transcripts led to the identification of 94 high-quality differentially
expressed sequences. Assembling analysis, plus
validation, rendered 65 candidate unigenes, organized as
14 contigs and 51 singletons. Thirty-four unigenes were
isolated from apomictic plants and 31 from sexual ones. A
total of 45 (69.2%) unigenes were functionally categorized.
While several of the differentially expressed sequences
appeared to be components of an extracellular receptor
kinase (ERK) signal transduction cascade, others seemed to
participate in a variety of central cellular processes like
cell-cycle control, protein turnover, intercellular signalling,
transposon activity, transcriptional regulation and endoplasmic
reticulum-mediated biosynthesis. In silico
mapping revealed that a particular group of five genes
silenced in apomictic plants clustered in a rice genomic
area syntenic with the region governing apospory in
Paspalum notatum and Brachiaria brizantha. Two of these
genes mapped within the set of apo-homologues in
genes mapped within the set of apo-homologues in
and Brachiaria brizantha. Two of these
genes mapped within the set of apo-homologues in
P. notatum. Four genes previously reported to be controlled
by ploidy were identified among those expressed differentially
between apomictic and sexual plants. In situ
hybridization experiments were performed for selected clones.
by ploidy were identified among those expressed differentially
between apomictic and sexual plants. In situ
hybridization experiments were performed for selected clones.
. Four genes previously reported to be controlled
by ploidy were identified among those expressed differentially
between apomictic and sexual plants. In situ
hybridization experiments were performed for selected clones.