MENZELLA Hugo Gabriel
Cloning and production of thermostable enzymes for the hydrolysis of steryl glucosides in biodiesel
AGUIRRE, ANDRES; PEIRU, SALVADOR; RASIA, RODOLFO M.; CASTELLI, MARÍA EUGENIA; HUGO G MENZELLA
METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)
Año: 2021 vol. 1
Vegetable oils derived biodiesels have a major quality problem due to the presence of precipitates formed by steryl glucosides, which clog filters and injectors of diesel engines. An efficient, scalable and cost-effective method to hydrolyze steryl glucosides using thermostable enzymes has been developed. Here, methods to discover, express in recombinant microorganisms and manufacture enzymes with SGase activity, as well as methods to treat biodiesel with such enzymes, and to measure the content of steryl glucosides in biodiesel samples are presented.