Supramolecular structures regulate beta-Gal functionality

SANCHEZ, JULIETA M; FLORES, SANDRA S.; CLOP, PEDRO D.; CLOP, EDUARDO M; NOLAN, VERONICA; PERILLO, MARÍA A.

Castilla

Congreso; Congreso Iberoamericano de Biofisica; 2018

Sociedad Iberoabericana de Biofisica

Supramolecular structures regulate β-Gal functionalityJM Sanchez Flores SS, Clop EM, Clop, PD Nolan, MV, and Perillo, MAIn our laboratory we studied the structure-function relationship of proteins in complex environment. We applied beta galactosidase from E. coli (β-Gal) as a model protein. In our work we proved that the soluble enzyme interacts with lipid interfaces. we demonstrated that in the presence of lipid interfaces the enzyme activity increased. In this conditions the structural features are related to a more hydrated but resistant to thermal unfolding structure respect to β-Gal in solution. We also prepared mixed β-Gal-phospholipid monolayer. At high surface pressure the AFM analysis showed a higher order oligomerization state of β-Gal at the lipid interface respect β-Gal tetramer.Actually we are overproducing a recombinant β-Gal In some condition we are able to produce β-Gal inclusion bodies (IBsβ-Gal) Interestingly, the β-Galpresentnot only higher activity respect to the soluble β-Galbut it also exhibit higher resistance to temperature and pH inactivation.In this context we conclude that the protein.protein contact at the lipid-water as the protein-water interfaces confers an activating environment towards protein allowing a non-native but stable structure.