Resolving phylogenetic history of Enterococcus mundtii using all enterococci core protein sequences

LUIS ESTEBAN; VÍCTOR S. BLANCATO; INGRID QUINTANA; CHRISTIAN MAGNI; MARTÍN ESPARIZ

Rosario

Congreso; 4to. Congreso Argentino de Bioinformática y Biología Computacional (4CAB2C) y 4ta. Conferencia Internacional de la Sociedad Iberoamericana de Bioinformática (SolBio); 2013

Asociación Argentina de Bioinformática y Biología Computacional y Sociedad Iberoamericana de Bioinformática

Enterococcus genera include commensal, environmental and human pathogen bacteria. For that reason, accurate identification is indispensable for both environmental and clinical studies. While phylogenetic trees based on comparative analysis of the 16S rDNA sequences is a standard identification and classification procedure, it was previously stated that some environmental strains of enterococci were incorrectly identified to the species level using this method [1]. E. mundtii CRL1656 is a pigmented bacterium that was isolated from stripping milk of an Argentinean cow and its genome was recently sequenced [2]. The aim of this study was to properly infer the phylogenic history of the strain. Reference [1] Nayak, B.S., Badgley, B, Harwood, V.J. Comparison of Genotypic and Phylogenetic Relationships of Environmental Enterococcus Isolates by BOX-PCR Typing and 16S rRNA Gene Sequencing. Appl Environ Microbiol 2011, 77(14):5050?5055 [2] Magni, C. et al: Draft genome sequence of Enterococcus mundtii CRL1656. J Bacteriol 2012, 194:550.