Human Amniotic Membrane Allograft: Comparison of two preservation methods in biocompatibility assays

LACERRA, CAROLINA; COMÍN, ROMINA; CID, MARIANA PAULA; LOPEZ LOZANO, TRISTAN; SCHMETS, DIEGO MAURO; IBARRA THEILER, RAÚL; MÁSCOLO, GERALDINE; SOBRERO, CECILIA BEATRIZ; SALVATIERRA, NANCY ALICIA

IFMBE PROCEEDINGS

Springer

Año: 2023

1680-0737

Tissue engineering is intended for the reconstruction of lost tissue due to destructive diseases such as inflammation and tumors, and requires three major elements: cells, signalling molecules and scaffolds. A scaffold is an extracellular artificial matrix with three dimensions; it is highly porous, 3D printed, and able to host and guide cells in their growth and in tissue regeneration. Currently, collagen hydrogels are the most popular material for cellular scaffolds, used successfully in clinical practice. Graphene oxide, graphene´s precursor in its synthesis, is a graphite oxide monolayer; even though the study of its properties for tissue engineering is still in its early stages, big results are expected. The project aims at designing, 3D printing and evaluating the features of collagen and graphene oxide scaffolds, for potential applications in tissue engineering. The ink obtained from these materials allowed loyalprintings, respecting structures and dimensions. The incorporation of graphene oxide in collagen scaffolds showed variations in their properties, keeping swelling ratio, decreasing porosity and solubility. Using a scanning electron microscope (SEM), we distinguished pure graphene oxide from collagen in the scaffold, where we observed the combination of both materials in micro-scale.