INVESTIGADORES
FONSECA maria isabel
congresos y reuniones científicas
Título:
Design of degenerated primers from bioinformatics online software for putative ACE-1 trancription factor in Peniophora sp
Autor/es:
ANA BELÉN RAMOS HRYB; MARIA ISABEL FONSECA; LAURA VILLALBA; PEDRO DARIO ZAPATA
Lugar:
Quilmes
Reunión:
Congreso; 1er Congreso Argentino de Bioinformática y Biología Computacional. online: http://www.slideshare.net/a2b2c/ramos-ace1; 2010
Institución organizadora:
Universidad Nacional de Quilmes
Resumen:
Cellulose-paper
industries have high environmental impact, that is why there are efforts to
reduce pollution. In this sense white rot fungi
such as Peniophora sp (BAFC 633) present enzymes with wide degradative
capacity, like Laccase (Lac) [1], with potential to alleviate environmental
problems. Several cupper-binding sites in Lac have been discovered [2] and the
presence of DNA sites coding transcription
factor in response to Cu+2 (ACE-1) in Phanerochaete chrysosporium (Genbank
accession number BF60559.1) has been demonstrated [3], which could activate the
laccase gene transcription [4]. The objective of this study was to define
conserved regions using bioinformatics tools and to design degenerated primers
to amplify a segment of a putative region coding ACE-1 in Peniophora sp. genome.
