Activation of PPAR a and PPAR b/d regulates Sertoli cell metabolism

REGUEIRA M; RIERA MF; GALARDO MN; PELLIZZARI EH; CIGORRAGA SB; MERONI SB

MOLECULAR AND CELLULAR ENDOCRINOLOGY.

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2014 vol. 381 p. 271 - 281

0303-7207

The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR a and PPAR b/d activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742 -pharmacological activators of PPAR a and PPAR b/d respectively. The fatty acid transporter CD36, carnitine palmitoyltransferase 1, long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases mRNA levels were analyzed. An increase in the above-mentioned genes in response to activation of both nuclear receptors was observed. Additionally, PPAR b/d activation increased lactate production as a consequence of increased pyruvate availability by inhibiting the Pyruvate Dehydrogenase Complex. Altogether, these results suggest that in SC, PPAR a activation participates in the regulation of FA metabolism. On the other hand, PPAR b/d activation regulates FA metabolism and lactate production ensuring simultaneously the energetic metabolism for SC and germ cells.