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ANCAROLA Mar√ća eugenia
congresos y reuniones científicas
Título:
Stage-dependent secretion of extracellular vesicles to the extra-parasite environment in the zoonotic cestode Echinococcus multilocularis
Autor/es:
MARIA EUGENIA ANCAROLA; JOHANNES HERBIG; LUCAS MALDONADO; SONJA HORVAT; ANTONIO MARCILLA; KRYSTYNA ALBRECHT; LAURA KAMENETZKY; KLAUS BREHM; MARCELA CUCHER
Lugar:
Buenos Aires
Reunión:
Workshop; International Society for Extracellular Vesicles Workshop EVs in Immunology; 2020
Institución organizadora:
International Society for Extracellular Vesicles
Resumen:
Echinococcosis is a worldwide zoonosis of public health concern caused by Echinococcus spp. These cestodes have an indirect life cycle in which the intermediate hosts (IH) become infected by ingesting eggs that develop into metacestodes (MC) mainly in the liver. The MC is a vesicle filled with hydatid fluid (HF) lined by an inner germinal layer that produces immature worms (protoscoleces-PS) and an outer acellular laminated layer. When the definitive host (DH) ingests PS, these develop into adults in the gut and release eggs in the host faeces. Echinococcus spp. show a long persistence in host tissues implying that parasites modulate the environment imposed by the host to allow their establishment, action that may involve extracellular vesicles (EV) transfer. Here, we analysed EV secretion in E. multilocularis to determine if EV may play a role in this interaction. For this, we analysed media conditioned by germinal layer primary cell cultures (PC), MC and PS, and also HF. Ultrastructural analyses showed the presence of EV-like structures in all the samples. Also, in MC tegument EV located in the interface between the laminated and germinal layers. Nanoparticle tracking analysis showed that EV concentration in MC was in the lower quantification limit, while in HF the concentration was ~20x higher. This suggests that MC EV may not be central in host-parasite interplay. Protein profiles assessed by LC-MS/MS showed a highly variable number of proteins identified, with only one protein detected in MC EV. Finally, hepatocytes were cultivated with labelled EV from PC and internalization was observed. In conclusion, EV may not be equally relevant in the host-parasite interplay throughout the life cycle of E. multilocularis since EV release depends on the morphology of the larval stages.