BECAS
ANCAROLA MarÍa eugenia
congresos y reuniones científicas
Título:
Cestode parasites secrete microRNAs packaged in extracellular vesicles
Autor/es:
ANCAROLA, MARÍA EUGENIA; MARCILLA, ANTONIO; HERZ, MICHAELA; MACCHIAROLI, NATALIA; PÉREZ, MATÍAS; ASURMENDI, SEBASTIÁN; BREHM, KLAUS; PONCINI, CAROLINA; ROSENZVIT, MARA; CUCHER, MARCELA
Lugar:
São Paulo
Reunión:
Workshop; ISEV Workshop on Cross-Organism Communication by Extracellular Vesicles: Hosts, Microbes, Parasites; 2016
Institución organizadora:
International Society for Extracellular Vesicles
Resumen:
Cestode parasites are platyhelminths passively transmitted among the hosts involved in their life cycle that can affect almost all vertebrate species. Zoonoses caused by cestodes are among the most severe neglected tropical diseases in humans prioritized by the World Health Organization. Most parasites have different strategies that allow them to regulate the host immune response and thus create a proper environment for their proper development and differentiation. Lately, it has increased the number of studies about extracellular vesicles(EV) secretion as a path of intercellular communication in many organisms including parasite-host relationships. In the case of helminth EVs, the presence of intravesicular RNA, in particular microRNAs, was confirmed as well as their internalization by host cells where they can modify the transcriptional profile.Since there are no reports on cestode EVs secretion, here we aimed to determine whether the larval stages of the model cestodes Taenia crassiceps and Mesocestoides corti secrete EVs and analyzed their protein and RNA content. First, we demonstrated the secretion of membrane-bound structures compatible with EVs by transmission electron microscopy. Then, we profiled the proteins carried by these structures by LC-MS/MS and identified many EVs markers found in eukaryote EVs. Finally, we proved that cestode EVs carry intravesicular small RNAs and particularly the presence of microRNAs was detected by RT-(q)PCR. This is the first report of EV secretion as well as microRNAs secretion in cestode parasites and could represent a parasite-parasite or parasite-host path of communication. Also, these results could contribute with relevant information for the future development of new diagnosis methods of these diseases through the detection of circulating parasite microRNAs as non-invasive biomarkers.