INVESTIGADORES
CUCHER Marcela Alejandra
congresos y reuniones científicas
Título:
Hydatid disease in genome era: comparative genomic analysis between Echinococuccus granulosus s.s. (G1 genotype) and Echinococcus canadensis (G7 genotype) draft genome
Autor/es:
MALDONADO L; ASSIS J; GOMES ARAUJO F; MACCHIAROLI N; CUCHER M; ROSENZVIT M; OLIVEIRA G; KAMENETZKY L
Reunión:
Congreso; 13th International Congress of Parasitology (ICOPA); 2014
Resumen:
BACKGROUND:
Echinococcus canadensis is a
platyhelminth parasite which keeps close phylogenetic relationship with Echinococcus granulosus and Echinococcus multilocularis members of
the class Cestoda that are involved in hydatid infections of humans and animals.
In South America three species of Echinococcus
have been reported E. granulosus s.s. (G1
and G2 genotypes), E. canadensis (G6
and G7 genotypes) and E. ortleppi (G5 genotype). Only limited genetic information
of E. canadensis G7 was reported so far. In this work we have
sequenced the genome of this species.
METHODS:
High quality genomic DNA has been extracted and paired-end libraries have been sequenced
by Illumina technology. Several
pipelines of assembly have been evaluated. The genome has been de novo assembled with Velvet using
different parameters until the best assembly was obtained. Also, reads have
been mapped over E. multilocularis reference
genome with BWA. Conserved genes have been annotated by CEGMA.
RESULTS:
Comparative studies have revealed high levels of nucleotidic identity between E. canadensis G7 with E. multilocularis as well as with E. granulosus s. s. G1. Interestingly, the procedure for in silico annotation employed in this
work allowed to identify more than 90% of highly conserved genes.
CONCLUSIONS: This is the
first report of E. canadensis G7 genome
obtained by high throughput sequencing, allowing a broad genome view of this
particular species.The knowledge of this new genome would provide information
for comparative genomics allowing to adapt prevention and diagnosis tools to
each epidemiological situation.