EFFECTS OF HYDROCARBONS ON CELL SURVIVAL AND CELL CYCLE OF BREAST TUMORIGENIC AND NON-TUMORIGENIC CELLS

MARIANA MARDIROSIAN; MARIEL NÚÑEZ; MARIANELA LASAGNA; TAMARA GALARZA; ANDRÉS VENTURINO; CLAUDIA COCCA

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Over the last centuries, contamination with polycyclic aromatic hydrocarbons (PAH) has risen due to the intensified industrial activities. PAH are a group of organic pollutants with adverse effects on humans and the environment, widely distributed in various ecosystems and present in crude oil, drinking water and food. Crude oil toxicity is mainly caused by the water-accommodated fraction (WAF). Our aim was to evaluate the effects of WAF and anthracene, one of the PAH present in WAF, on cell survival and proliferation, cell cycle and the antioxidant system. MCF-7 and MDA-MB-231 human breast tumorigenic cells and/or MCF-10A epithelial non-tumorigenic cells were exposed for 7d to WAF (serial dilutions from 1/500 to 1/25) or anthracene (0 to 28 μM) to perform clonogenic assays, for 72h to study viability (MTT assay), glutathione S-transferase (GST) and catalase (CAT) activities and for 18h to study cell cycle by flow cytometry. Neither WAF nor anthracene affected MCF-7 and MDAMB-231 clonogenicity. However, anthracene decreased MCF-10A proliferation in a dose-dependent manner (p