Further studies on vertebrate S-type lectins: cross-reactivity between toad and human lectins

ELOLA, M. T.; FINK, N. E.

BIOLOGICAL RESEARCH

SOC BIOLGIA CHILE

Lugar: Santiago de Chile; Año: 1997 vol. 30 p. 99 - 104

0716-9760

Galectins (S-type or S-Lac lectins) are a well-defined family of beta-galactoside animal lectins characterized by a high sequence homology in the carbohydrate-binding domain. We have previously purified and characterized the S-type lectin from the ovary of the toad Bufo arenarum. In this study, we purified the S-type lectins from Bufo arenarum ovary and human spleen by an improved method which included ion exchange and affinity chromatography. Antibody cross-reactivities between both lectins and some other S-type lectins showed that they share epitopes. Glycosylation studies carried out with detection/differentiation kits suggested that both lectins are not glycosylated.