Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans

M. ALEJANDRA MARTINEZ; OSVALDO D. DELGADO; MARIO D. BAIGORI; FAUSTINO SINERIZ

BIOTECHNOLOGY LETTERS

SPRINGER

Lugar: The Netherlands; Año: 2005 p. 545 - 550

0141-5492

AbstractThe BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-b-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg-1.