Tissue culture for in vitro germplasm conservation of Arachis spp.

LUIS AMADO MROGINSKI; HEBE YOLANDA REY; MARÍA LAURA VIDOZ

Congreso; Reunión Anual de la American Peanut Research & Education Society; 2000

In vitro tissue culture offers procedures of overcoming various problems in conservation of crop genetic resources. The development of methods for the storage of in vitro cultures may resolve the problems, which normally occur in the germplasm conservation of certain vegetatively propagated species of Arachis and those producing few seeds. One method for short and medium term storage is based in inducing slow growth of the cultures. Both, meristems or nodal segments of A. correntina and A. pintoi (2n=2x=20 and 2n=3x=30) were cultured on MS medium and incubated for eight months in 4 C. High frequency of plant survival were obtained. Cryopreservation for long term storage of Arachis spp requires further research and development. techniques based on using somatic embryos of A. pintoi and A. glabrata are in progress.