In vitro plant regeneration of Arachis correntina (Leguminosae) through somatic embryogenesis and organogenesis

MARÍA LAURA VIDOZ; PABLO KLUSACEK; HEBE YOLANDA REY; LUIS AMADO MROGINSKI

PLANT CELL TISSUE AND ORGAN CULTURE

SPRINGER

Año: 2006 vol. 86 p. 111 - 115

0167-6857

In vitro protocols for plant regeneration of Arachis correntina through both somatic embryogenesis and organogenesis were developed using immature leaves as explants. Morphologically normal somatic embryos were obtained on culture media composed of 20.70 or 41.41 µM picloram (PIC) with the addition of 0.044 µM 6-benzylaminopurine (BA), resulting in a 33% and 24% of conversion into plants respectively. The source of explants and the developmental stage of the leaves had a marked effect on somatic embryogenesis. The second folded immature leaves from in vitro growing plants were the most responsive producing up to 30% embryogenesis in MS + 41.41 µM PIC.  Embryos converted into plants after transfer to MS medium devoid of growth regulators and these plants were successfully acclimatised. Adventitious shoots were obtained on culture media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthaleneacetic acid  (NAA) with or without 0.044 µM BA, achieving plant regeneration in the induction media. The highest percentage of bud formation was obtained on culture medium composed of MS + 10.74 µM NAA + 0.044 µM BA (12.5%). Roots were formed on all culture media tested. Regenerated plants were transferred to pots and grew well under greenhouse conditions.