TNFRP55 SIGNALING CONTROLS INFLAMMATORY RESPONSE INDUCED BY STIMULATION WITH TLR AGONISTS

JERÉZ BELÉN; ELIÇABE R. JAVIER; JURI AYUB MAXIMILIANO; DI GENARO MARÍA SILVIA

LIMA

Congreso; X CONGRESO DE LA SOCIEDAD LATINOAMERICANA DE INMUNOLOGÍA ALAI 2012; 2012

ASOCIACIÓN LATINOAMERICANA DE INMUNOLOGIA

TNF plays a key role in inflammation. However, new evidence suggests an anti-inflammatory role for TNF. The mechanisms involved in this effect are unknown. TNFRp55 is the receptor implicated in most TNF effects. In the present work, we studied the impact of TNFRp55 deficiency in macrophages (MΦ) stimulated by a set of agonists to mouse TLR1-9 or Yersinia LPS. We investigated the modulation on nitric oxide (NO) pathway and IL-6 and IL-12/23p40 responses. In addition, we studied the impact of Yersinia LPS stimulacion on IL-12/23p40 mRNA expression and stability. Peritoneal MΦ obtained from C57BL/6 wild-type (WT) and TNFRp55-/- mice were incubated without or with the TLR agonists. Nitrite production in the supernatants was measured using the Griess reagent and the cytokines by ELISA kits. We found higher levels of NO and IL-6 in TNFRp55-/- than WT MΦ stimulated to LPS from E. coli K12 (p