Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex

MAXIMILIANO JURI AYUB; CRISTIAN SMULSKI; BENSON NYAMBEGA; NATALIA BERCOVICH; DAN MASIGA; MARTIN VAZQUEZ; CARLOS AGUILAR; MARIANO LEVIN

GENE

Elsevier

Año: 2005 vol. 357 p. 129 - 136

0378-1119

The large subunit of the eukaryotic ribosome possesses a long and protruding stalk formed by the ribosomal P proteins. Four out of five ribosomal P proteins of Trypanosoma cruzi, TcP0, TcP1a, TcP2 a, and TcP2b had been previously characterized. Data mining of the T.cruzi genome data base allowed the identification of the fifth member of this protein group, a novel P1 protein, named P1b ƒ|ƒnTo gain insight into the assembly of the stalk, a yeast two-hybrid based protein interaction map was generated. A parasite specific profile of interactions amongst the ribosomal P proteins of T. cruzi was evident. The TcP0 protein was able to interact with all both P1 and both P2 proteins. Moreover, the interactions between P2b with P1a as well as with P2a were detected, as well as the ability of TcP2b to homodimerize. A quantitative evaluation of the interactions established that the strongest interacting pair was TcP0-TcP1b.