Effect of cholinergic stimulation on ovarian physiology ganglionic in the first proestrus in the rat

DELSOUC MB; CARRIZO D; DELGADO SM; RASTRILLA AM

San Juan

Congreso; 2da Reunión Conjunta de Sociedades de Biología de la República Argentina; 2011

Sociedad Argentina de Biología junto con la Sociedad de Biología de Cuyo y la Sociedad de Biología de Córdoba

It recognized the involvement of nitric oxide (NO) in the ovaric steroidogenesis. Using the ex vivo coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system, has been shown to stimulating the ganglion with cholinergic agents, modify the release of NO and progesterone (P) ovarian in prepubertal, pregnancy and adult rats. The aim of this work was to study in the same experimental scheme, if CG-cholinergic agents modify the release of NO and P, and the expression of the enzymes 3β-HSD and 20α-HSD (of synthesis and degradation of P respectively) in the Proestrus stage in the first estrous cycle. The system was incubated in Krebs Ringer at 37ºC. The working groups were: a) control b) cholinergic agonist acetylcholine (Ach), c) muscarinic antagonist atropine (Atr) and d) nicotinic antagonist hexamethonium (Hex) all in 10-6M concentration. We determined NO (Griess technique) and P (RIA) in the ovarian compartment at 15´, 30´, 60 ´and 120´. The expression of enzymes was determined by RT-PCR, in the ovary to 120´. ANOVA 1 followed by Tukey test with a statistical significance of p