The cholinergic agents modify the luteal regression at the first diestrous in the rat

DELSOUC B ; VALLCANERAS S; BENEGAS J; CARRIZO D; DELGADO SM; RASTRILLA AM

Mendoza

Congreso; XXVIII Annual Scientific Meeting; 2010

Sociedad de Biología de Cuyo

Using the ex vivo coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system, we have shown that the CG stimulation with cholinergic agents, modifies the secretion of ovarian steroids in rats at different stages of the reproductive life. The aim was to elucidate the effect of Acetylcholine (Ach), as cholinergic agonist, Atropine and Hexametonium, as muscarinic and nicotinic antagonist respectively in CG on the luteal regression at the first diestrous of estrous cycle. The system was incubated in Krebs Ringer-glucose at 37ºC. The cholinergic agents (all in 10-6M concentration), were added in the CG compartment. Controls were not stimulated. Release of ovarian Progesterone (P) was measured by RIA at 15, 30, 60 and 120 min. At 120 min, ovarian mRNA expression of bax and bcl-2 (apoptosis regulators) were assessed by RT-PCR. ANOVA 1 followed by Tukey test with a statistical significance of p