INVESTIGADORES
LUQUET carlos Marcelo
congresos y reuniones científicas
Título:
Accumulation and biochemical effects of microcystin-LR on Patagonian silverside (Odontesthes hatcheri) fed with Microcystis aeruginosa cells
Autor/es:
LUQUET, CARLOS M; BIECZYNSKI, FLAVIA; BIANCHI, VIRGINIA A
Lugar:
San Diego CA
Reunión:
Conferencia; Experimental Biology 2012; 2012
Institución organizadora:
FASEB
Resumen:
Accumulation and biochemical effects of microcystin-LR on
Patagonian silverside (Odontesthes hatcheri) fed with
Microcystis aeruginosa cells
Carlos Marcelo Luquet, Flavia Bieczynski, Virginia Angélica
Bianchi. INIBIOMA, CONICET, Junín de los Andes, Argentina
We studied accumulation and biochemical effects of microcystin-
LR (MC) in the Patagonian silverside Odontesthes hatcheri after
dietary administration of the cyanobacteria Microcystis aeruginosa
(load~1.3 μg MC / g fish, incorporated in standard fish food).
After 12 h, MC content in liver did not differ between fish fed with
crushed or intact cells, demonstrating O. hatcheris capacity to
digest cyanobacterial cells and also absorb free MC.
In a second experiment, fish received crushed toxic cells, non-toxic
cells or control feed; MC accumulation was monitored for 48 h.
Protein phosphatase 1 (PP1), catalase (CAT) and glutathione S
transferase (GST) activities and lipid peroxidation (MDA) were
measured in liver and intestine. Methanol-extractable MC was
determined by PP1 inhibition assay, total MC (extractable +
protein-bound) was measured by Lemieux oxidation - gas
chromatography / mass spectrometry. MC accumulated rapidly up
to 22.9 and 9.4 μg MC / g in intestine and liver respectively,
followed by a decreasing tendency. Protein-bound MC represented
65-98 % of total MC in both tissues. CAT and GST activities and
MDA were significantly increased by MC in liver, but not in
intestine. We conclude O. hatcheri is able to digest cyanobacteria,
accumulating MC mostly bound to proteins. This could have
implications for aquaculture and trophic chain toxic transference.
Grants: CONICET PIP 0282, ANPCYT PICT 0021