Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus

LUQUET, C.M.; WEIHRAUCH, D; SENEK, M.; TOWLE, D.W.

JOURNAL OF EXPERIMENTAL BIOLOGY

The Company of Biologists

Lugar: Londres; Año: 2005 vol. 208 p. 3627 - 3636

0022-0949

Using quantitative real-time PCR, the expression of mRNAs encoding three transport-related proteins and one putative housekeeping protein was analyzed in anterior and posterior gills of the euryhaline crab Chasmagnathus granulatus following transfer from isosmotic conditions (30‰ salinity) to either dilute (2‰) or concentrated (45‰) seawater. Modest changes were observed in the abundance of mRNAs encoding the housekeeping protein arginine kinase and the vacuolar-type H+-ATPase Bsubunit, both of which were highly expressed under all conditions. By contrast, the expression of Na+/K+-ATPaseChasmagnathus granulatus following transfer from isosmotic conditions (30‰ salinity) to either dilute (2‰) or concentrated (45‰) seawater. Modest changes were observed in the abundance of mRNAs encoding the housekeeping protein arginine kinase and the vacuolar-type H+-ATPase Bsubunit, both of which were highly expressed under all conditions. By contrast, the expression of Na+/K+-ATPasefollowing transfer from isosmotic conditions (30‰ salinity) to either dilute (2‰) or concentrated (45‰) seawater. Modest changes were observed in the abundance of mRNAs encoding the housekeeping protein arginine kinase and the vacuolar-type H+-ATPase Bsubunit, both of which were highly expressed under all conditions. By contrast, the expression of Na+/K+-ATPase+-ATPase Bsubunit, both of which were highly expressed under all conditions. By contrast, the expression of Na+/K+-ATPase+/K+-ATPase -subunit mRNA and Na+/K+/2Cl– cotransporter mRNA was strongly responsive to external salinity. During acclimation to dilute seawater, cotransporter mRNA increased 10–20-fold in posterior gills within the first 24·h while Na+/K+-ATPase -subunit mRNA increased 35–55- fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96·h and Na+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.·h while Na+/K+-ATPase -subunit mRNA increased 35–55- fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96·h and Na+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.+/K+-ATPase -subunit mRNA increased 35–55- fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96·h and Na+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.·h and Na+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.-subunit mRNA and Na+/K+/2Cl– cotransporter mRNA was strongly responsive to external salinity. During acclimation to dilute seawater, cotransporter mRNA increased 10–20-fold in posterior gills within the first 24·h while Na+/K+-ATPase -subunit mRNA increased 35–55- fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96·h and Na+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.·h while Na+/K+-ATPase -subunit mRNA increased 35–55- fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96·h and Na+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.+/K+-ATPase -subunit mRNA increased 35–55- fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96·h and Na+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.·h and Na+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.+/K+-ATPase -subunit increased approximately 25- fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.