INVESTIGADORES
FALCONE FERREYRA Maria lorena
congresos y reuniones científicas
Título:
UV-B REGULATION OF MAIZE FLS1 AND FNS ENZYMES
Autor/es:
EMILIANI, J.; FALCONE FERREYRA, M.L.; GROTEWOLD, E; CASATI, P.
Lugar:
Tucumán
Reunión:
Congreso; XLV Reunión anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; 2009
Resumen:
Flavonoids are a highly diverse class of secondary plant metabolites
found in vascular plants. These compounds have a significant
impact in plant biology and benefits for human health. One group of
these, the flavones, are synthesized from flavanones by the enzyme
flavone synthase (FNS). Other flavonoids, the flavonols, are
synthesized from the dihydroflavonols by the action of flavonol
synthase (FLS). Here, we investigated the regulation of ZmFLS1 and
a putative ZmFNS by UV-B radiation in five maize land races from
high altitudes and in the low altitude lines B73 and W23. ZmFLS1
and ZmFNS are induced by UV-B in most lines, demonstrating that
these genes are under UV-B control. To relate the induction of
ZmFLS1 and ZmFNS by UV-B with its regulation by the P1 or
C1/PL+R/B flavonoid regulators, we investigated whether the
expression of these transcription factors is also UV-B regulated. UVB
significantly increases P1 transcripts in all lines, being almost
undetectable under the control conditions. In the absence of UV-B, B
and PL1 are expressed at variable levels in the different lines. After
UV-B exposure for 8h, B and PL1 showed an increase in their
expression in most lines. Thus, in response to UV-B, the upregulation
of ZmFLS1 and ZmFNS is possibly mediated by P1 and
PL1+B, which are also induced by UV-B.