Evaluation of the toxic effect of Zn/Cd on the antioxidant response in Glycine max and probable competition with Ca/Mg transporters

CV GATICA AGUILAR; LV GALLARDO; ME CIMINARI; N CASIN; P PACHECO; LB PENA; RA GIL; MV PÉREZ CHACA

Mendoza

Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2022

Sociedad de Biología de Cuyo

Heavy metals pollution has produced big soil problems all around the world. Cadmium (Cd) is a heavy metal without biological functions and also causes harmful effects at cellular and molecular levels. Zinc is an essential microelement for plants, but in excessive concentrations it causes alterations in physiological, biochemical and molecular processes. There are soil ions that interact with Zn and possible pollulant Cd, such as Calcium (Ca) and Magnesium (Mg). Metals compete to get inside, transporting and utilization in the plant; because they have similar chemicals characteristics and also plants absorbed them using the same transporters (IRT1-ZIP). The study objective was to determine competition between transporters and their inside circulation among Cd, Zn and ions like Ca and Mg in Glycine max leaves as far as evaluating this interactions influences in antioxidant and prooxidant parameters. Leaves were obtained after 10 days of plant development under hydroponics in Hoagland´s nutrient solution conditions and subjected to contamination with two ions (Zn and Cd) during 6 days. The ZnCl2 concentrations used in the study were: 0, 0.6 and 4.8 mM and 40μM of CdCl2, as a constant concentration. Morphophysiological parameters were measured: photosynthetic pigments (Chlor) and carotenes (Car), Ca and Mg endogen content, CAT and APX antioxidant enzymes, MDA and H2O2 content were measured as oxidative stress parameters. Results showed a significant decrease in Car and Chlor in the last treatments with Zn (4.8 mM)/Cd (p