INVESTIGADORES
MARTINEZ PERALTA Liliana A.
artículos
Título:
Characterization of a virus variant produced by L cells persistently infected with lymphocytic choriomeningitis virus.
Autor/es:
C.STOCKER, L.MARTÍNEZ PERALTA, T.KRATZBERG, F. LOHMANN, M. BRUNS.
Revista:
JOURNAL OF GENERAL VIROLOGY
Editorial:
The Society for General Microbiology,
Referencias:
Lugar: Reading, Inglaterra.; Año: 1994 p. 3431 - 3439
ISSN:
0022-1317
Resumen:
Continuous cultivation of murine L cells infected with
lymphocytic choriomeningitis virus strain Armstrong
leads to production of L(Arm) cells, which produce a
predominantly cell-associated attenuated variant, the
L(Arm) virus. The relatively few infectious particles that
are released have lost the ability to form plaques on L
cells and to cause illness in mice even if inoculated
intracerebrally. Based on equal protein MrS, antigenicity
and protein kinase activity, essentially identical results
were obtained for the purified Armstrong and L(Arm)
viruses. There was also no difference in production and
release of particles with the potential to cause homologous
interference. Such particles consisted of two
types, one of which was highly susceptible to u.v.-
irradiation, the other was highly resistant. In the case of
the L(Arm) virus interfering particles, it appears that the
u.v.-irradiation-susceptible forms represented infectious
virus. Purified L(Arm) virus particles contained considerable
quantities of subgenomic forms of (small) S- and
(large) L-RNA and their complementary counterparts,
which all appeared to be replicated autonomously in an
unenriched manner.