Evaluación de la inmunofluorescencia indirecta como prueba suplementaria en el diagnóstico de la infección por HIV-1

A.CEBALLOS, C.DEVITO, S. PAMPURO, O.LIBONATTI, L. MARTÍNEZ PERALTA.

REVISTA ARGENTINA DE MICROBIOLOGíA

Asociación Argentina de Microbiología.

Lugar: Buenos Aires, Argentina.; Año: 1998 vol. 30 p. 59 - 63

0325-7541

In order to be used as an alternative or complementary test to confirm HIV-1 infection, the efficiency of indirect immunofluorescence assay (IFA) was compared with Western blot (WB) in 362 samples from persons with high and low risk behaviour. A panel of sera with 220 WB positive, 122 WB negative and 20 WB indeterminate sera were tested by an "in house" IFA. The sensitivity of IFA was found to be 98.63% and the specificity 98.36%. Therefore, IFA appeared to be an efficient alternative method to WB, since the cost of testing by IFA is less than 10% of WB testing. We observed a direct relationship between WB protein reactivity and IFA results. In 15 samples with coincident indeterminate results for WB and IFA, antibody reactivity to p24 and gp160 presented the highest frequency. On the other hand, antibodies to viral glycoproteins were always present in IFA weak positive samples, showing their high predictive value.