Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus niger LBM 134.

DÍAZ GABRIELA VERÓNICA; CONIGLIO ROMINA OLGA; ORTELLADO LAURA ESTER; ZAPATA PEDRO DARÍO; MARÍA ALEJANDRA MARTÍNEZ; FONSECA MARÍA ISABEL

Food Production, Processing and Nutrition

Springer Nature

Año: 2024

2661-8974

Agricultural industries search for biotechnological alternatives for their waste management because they mean a significant concern and environmental challenge that can be used for obtaining value-added compounds. For that, the principal objective of this work was to release bioactive compounds from citrus waste using a homemade enzymatic cocktail of A. niger LBM 134. This fungal enzymatic cocktail was produced using sugarcane bagasse as substrate, which increased the ecological sustainability and the cost-effectiveness of the bioprocess. The β-glucosidase activity of this cocktail was stable at 50 °C and kept 30% of its residual activity even after treatment at 100 °C for 5 min. For the first time, we reported a technique that evidenced a β-glucosidase electro morpho profile in polyacrylamide gels using 4-methyl umbelliferyl glucoside as the substrate. This cocktail was efficiently employed to extract the phenolics hesperetin, quinic, ρ-coumaric, and gallic acid, and the bioactive amino acid tryptophan from citrus waste, achieving the highest values of phenolics, approximately 112% and 30% more than conventional and commercial enzyme extraction, respectively. We valorize two agricultural wastes, sugarcane bagasse, and citrus waste, for obtaining high added-value products such as enzymes and compounds with multiple biological properties.