Location study of ermTR gene in Streptococcus agalactiae

NOVOSAK MG; MOYA ALVAREZ A; BOBADILLA FJ; CORTESE IJ; LIOTTA J; LACZESKI M

Mar del Plata

Congreso; SAIC.SAI.SAFE.2016; 2016

Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE)

Streptococcusagalactiae (GBS) is the leading cause of severe invasiveinfections in infants less than three months. Meningitis, pneumonia and sepsisare the leading cadres in these children. These infections are described as themost serious that an individual may suffer in their first hours of life, thematernal transmission is the main route of infection (95%). Although intrapartum antibiotic prophylaxis (IAP) with penicillincan significantly decrease neonatal GBS diseases, rates of resistance toantibiotics recommended for pregnant women allergic to penicillin, such asclindamycin and erythromycin, have increased. Among the mechanisms ofresistance are changes in the target site mediated by erm genes frequently associated with transposons (conjugative andnon-conjugative) on chromosome or plasmid. The genomic location of these genesis relevant for its potential interspecific horizontal transmission. The aim ofthis study was to determine the location of ermTRgene, one of the most reported in GBS. Three strains of GBS were selected fortheir history of phenotypic resistance and the presence of ermTR gene. The identification of GBS strains was confirmed byconventional biochemical tests. The extraction and purification of plasmid,cromosomal and total DNA were performed with commercial kits. The extractioncontrol and/or chromosomal plasmids contamination was monitored by PCR withtarget in the 16S gene. Assayslocation of ermTR gene was performedusing specific PCR. In the development of PCR for ermTR gene amplification a single sharp band of the expected size wasobtained. Location assays show the presence of this ermTR gene into the chromosomal DNA of all the strains. Inconclusion, the chromosomal location of the ermTRgene was determined and is necessary to continue with further studies ofgenomic context to contribute to the knowledge of resistance to macrolides andlincosamides.