Yersinia enterocolitica YopH-deficient strain increases neutrophil recruitment by modulation of CXCR2 expression

DAVE MN; SILVA JE; JERÉZ MB; ARCE ME; FILIPPA VP; ELIÇABE J; GORLINO C; DI GENARO MS

Buenos Aires

Congreso; la LXIII Reunión Anual de SAI en el marco del Primer Congreso Internacional conjunto (I Meeting LASID-SAI-FAIC) junto a LASID (IV Meeting LASID) y FAIC(FAIC-II); 2015

Sociedad Argentina de Inmunlogia

YopH is a tyrosine phosphatase critical for virulence of Yersinia enterocolitica (Ye).We have demonstrated that Ye deficient in YopH (YedyopH) increases neutrophil recruitment in Peyer´s patches (PP). The chemokines CXCL1 and CXCL2 by signaling predominantly through CXC receptor 2 (CXCR2) are crucial for the neutrophil migration. The purpose was to analyze the expression of these chemokines in PP after Ye yopH infection. Methods: C57BL/6 mice were orally infected with YedyopH or with the wild-type strain Ye WA-314 (1-5 108CFU/mouse). At day 3 after infection, PP were aseptically removed and bacterial counting was performed. CXCL1 was quantified in the homogenates using the CBA Assay and the level of CXCL2 was measured by ELISA. The expression of CXCL1 mRNA was studied by qPCR analysis. The CXCR2 expression as well as neutrophil number in blood and PP were analyzed by flow cytometry. Results. Neutrophil recruitment in PP resulted augmented after YedyopH infection (p