Contribution of IL-17 in the immune response to Yersinia enterocolitica.

DAVE MN; DIAZ-GABUTTI S; ELIÇABE J; GORLINO C; DI GENARO MS

Los Cocos, Cordoba

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2013

Sociedad Argentina de Inmunología (SAI)

Yersinia outer proteins (Yop) are important virulence factors used by Yersinia enterocolitica (Ye) to evade the immune response and promote its survival in host tissues. YopH is a powerful protein-tyrosin phosphatase that contributes to bacterial evasion. The objective was to elucidate the role of IL-17 in the immune response against Ye wild-type and yopH deletion mutant (Ye ΔyopH). For this purpose, C57BL/6 wild-type (WT), IL-17R-/- and TNFRp55-/- mice were orally infected with 1-5×108 Ye WA-314 or with Ye ΔyopH. Survival during 21 days after infection, and number of colony forming units (CFU)/organ) at day 7 were determined. Weight loss was registered during 7 days. Moreover, IL-17 secreted by splenocytes obtained 5 days after infection and re-stimulated with heat-killed Yersinia (HKY) was determined by ELISA and compared with mice that received phosphate buffered saline (control). Ye ΔyopH infection decreased mortality of both WT and IL-17R-/- mice compared to Ye WA-314 infection (2- and 3-fold, respectively). In contrast with Ye ΔyopH infection, CFU number in mesenteric lymph nodes and spleen of TNFRp55-/- and IL-17R-/- mice after Ye WA-314 infection were higher compared to WT mice ((p