TNFRp55 signaling modulates circadian innate immune response.

ARIAS, JOSE L; JEREZ, MARIA B; DI GENARO, MARIA S

Los Cocos, Cordoba

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2013

Sociedad Argentina de Inmunología (SAI)

The immune system is regulated by circadian mechanisms. The pro-inflammatory cytokine production by macrophages in response to LPS is determined by the intrinsic circadian clockwork. On the other hand, TNF was shown to induce an ?inflammatory clock gene response? (Cavadini et al, 2007). However, the cross-talk between the circadian and immune systems is largely unknown. Our objective was to study the consequences of TNFRp55 deficiency on the circadian production of pro-inflammatory mediators by LPS-stimulated macrophages. Thioglycolate-elicited peritoneal macrophages from six C57BL/6 wild-type (WT) and TNFRp55 knockout (KO) mice were harvested, pooled and plated for tissue culture. Cells were synchronized by serum shock for two hours. Cultures were stimulated every 6 hours with Yersinia LPS in a 48h-period. Supernatants were collected thereafter, and IL-6 and IL-12 production was assessed by ELISA, and nitrites by Griess? method. We found that IL-6, IL-12 and nitrites present a temporal variation (one-way ANOVA p