TNFRp55 deficiency impacts on dendritic cell phenotype in Yersinia-induced reactive arthritis.

SILVA JE ; ELIÇABE J ; DAVE MABEL ; DI GENARO MS.

Cordoba

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2013

Sociedad Argentina de Inmunología (SAI)

Reactive arthritis (ReA) is a particular type of arthritis originated from certain gastrointestinal or genitourinary infections. In previous studies, we reported the development of chronic Yersinia enterocolitica (Ye)-induced ReA in mice lacking TNF receptor (TNFR) p55. Dendritic cells (DCs) exist as phenotypically distinct subsets localized in different microenvironments. The role of DCs in this chronic arthritis model is unclear. The purpose was to compare the phenotype of DCs from joint regional lymph nodes (RLNs) and mesenteric lymph nodes (MNLs) of TNFRp55-/- and C57BL/6 wild type (WT) mice. Furthermore, we studied whether TNFRp55 deficiency influences CD80 and MHCII up-regulation on bone marrow derived?DCs (BM-DCs) from both mouse groups after Ye LPS stimulation. WT and TNFRp55-/- mice were orally infected with 1-5 × 108 Ye O:3 MHC 700 strain. Twenty-one days after infection, a single-cell suspension was prepared from MNLs and RLNs and the phenotype of DCs was analyzed by flow cytometry. Moreover, MHCII and CD80 expression was studied in TNFRp55-/- and WT BM-DCs 24 h after stimulation with Ye LPS (1μg/ml). TNFRp55-/- mice showed a significant increase in the absolute number of total DCs, and in CD11b+ subpopulation in RLNs (p