CYTOKINE PRODUCTION IN MESENTERIC LYMPH NODES INDUCED BY Yersinia enterocolitica INFECTION: ROLE OF YOPH

DÍAZ MS ; DAVE MN; ELIÇABE RJ; CARGNELUTTI E; SILVA JE ; DI GENARO MS

Potrero de los Funes, San Luis

Congreso; XXX Reunión anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biología de Cuyo

Yersinia enterocolitica (Ye) is an enteropathogenic bacterium that invades Peyer patches (PP) and may disseminates to mesenteric lymph nodes (MLN), spleen, lung, and liver. YopH, is a tyrosine phosphatase secreted by Ye that inhibits phagocytosis and lymphocyte activation. The role of YopH during in vivo infection have not been completely elucidated. The cytokine IFN- plays a central role in immune response against Ye. The aim was to compare IFN- production in MLN after oral infection with Ye WA-314 (wild-type) or YeyopH (YopH deficient). Moreover, we analized the production of IL-17, a cytokine associated with mucosal immune response. Female 6-8-week-old C57BL/6 mice were orally infected with 1?5 108 cfu of Ye.The bacterial number in PP, MLN and spleen were determined 5 days after infection. Intracellular cytokine production was analysed by flow cytometry in MLN cell suspensions re-estimualted in vitro with heat-killed Yersinia (HKY). We detected lower number of CFU in PP and MLN after YeyopH infection (p