EFFECT OF TNFRP55 DEFICIENCY ON THE CIRCADIAN LOCOMOTOR ACTIVITY AND THE ESTROUS CYCLE IN MOUSE

RAGUSA JA; ANZULOVICH AC; CASAIS M; DELGADO SM; DI GENARO S; ANZULOVICH AC

Mendoza

Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

The master clock in the suprachiasmatic nucleus synchronizes peripheral clocks through diffusible signals and behavioural rest-activity cycles. However, such signals are not still completely elucidated. It is known that the TNF suppresses clock genes expression. Our objective was: to analyze the effect of TNFRp55 deficiency on the clock-controlled rhythmic locomotor activity as well as on the day-night variation of the estrous cycle in C57BL/6 mice. Circadian locomotor activity was registered during 20 days using the Archron system. Analysis and characterization of the estrous cycles were performed on vaginal smears obtained during 12 days at ZT2 (9h) and ZT14 (21h). The data were analysed by Mann-Whitney test, significance p<0.05. We observed nocturnal activity decreased and desynchronized in the TNRFp55-/- mice. Estrous cycles last 6,0±0,6 vs 5,15±0,5 (p<0.03) days in wild type and TNRFp55-/- mice, respectively. TNF regulates the endogenous clock activity and the daily occurrence of the estrous cycle through its TNFRp55TNFRp55 deficiency on the clock-controlled rhythmic locomotor activity as well as on the day-night variation of the estrous cycle in C57BL/6 mice. Circadian locomotor activity was registered during 20 days using the Archron system. Analysis and characterization of the estrous cycles were performed on vaginal smears obtained during 12 days at ZT2 (9h) and ZT14 (21h). The data were analysed by Mann-Whitney test, significance p<0.05. We observed nocturnal activity decreased and desynchronized in the TNRFp55-/- mice. Estrous cycles last 6,0±0,6 vs 5,15±0,5 (p<0.03) days in wild type and TNRFp55-/- mice, respectively. TNF regulates the endogenous clock activity and the daily occurrence of the estrous cycle through its TNFRp55