Expression of pro-metalloproteinase-1 in human spondyloarthropathy fibroblast-like synoviocytes by stimulation with Yersinia enterocolitica antigens

LACOSTE M.G; CARGNELUTTI E.; VILLARREAL R; TAMASHIRO H; DI GENARO M.S

Viña del Mar, Chile

Congreso; 9th Latin American Congress of Immunology (Immunochile 2009); 2009

Sociedad Latinoamerica de Inmunología

Spondyloarthropathies are a group of chronic disorders with articular tissue destruction. We investigated the effect of Yersinia antigens on MMP-1 (fibroblast collagenase) induction in human spondyloarthropathy fibroblast-like synoviocyte (FLS). FLS were isolated from synovial fluid of a patient with undifferentiated spondyloarthropathy, and used after passages 3 to 7. FLS were stimulated with heat killed Yersinia (HKY: 107 or 108 bacteria/ml), Yersinia lipopolyssacharide (LPS: 100 ng/ml) or zymozan (10 ug/ml), as a control, for 24, 48 and 72 h. MMP-1 activity and expression in culture supernatants were analyzed by casein zymography and Western blotting, respectively. The casein zymography yielded two bands (58 and 50 kDa) with proteolytic activitiy after HKY or LPS stimulation for 48 and 72 h. The induction of MMP-1 was confirmed by Western blot since a specific band corresponding to pro-MMP-1 was observed after HKY or LPS stimulation at the mentioned times. We concluded that Yersinia antigens induce pro-MMP-1 in spondyloarthropathy FLS and suggest that bacterial components in the joint could play a role in the pathogenesis and activation of spondyloarthropathies. Understanding the joint destructive process and identifying of MMPs are keys to the future treatment development.