Effect of Tumor Necrosis Factor receptor p55 deficiency on the severity of post-Yersinia reactive arthritis

CARGNELUTTI D; CASTRO D; IANIZZOTTO G; VALDEZ S,; GUZMÁN A,; DI GENARO S.

Córdoba, Argentina

Congreso; VII Congreso Latinoamericano de Inmunología; 2005

Asociacion Latinoamericana de Inmunología (ALAI)

Tumor necrosis factor (TNF)-a is associated with protection against Yersinia. TNF receptor (TNFR) p55 is though to be the dominant pathway in the majority of known TNF effects. The objective of the present work was to study the role of tumor necrosis factor receptor (TNFR) p55 in the Yersinia-associated arthritis by the use of a murine model of oral Yersinia infection. The expression of cytokine, TLR, Bcl-2 and Bax mRNA in mesenteric lymph nodes (MLN) and joints. TNFRp55 deficient (TNFRp55-/-) and C57BL/6 mice were orally infected with 1x107 colony-forming units (CFU) of  Y. enterocolitica serotype O:3. Mice were daily observed and arthritis development was monitored by clinical score, joint assessment and x-ray. Mice were sacrificed at 3, 39 or 57 days post-infection (p.i.) for examination of histopathology of joints and bacterial clearance. The results indicates that TNF signaling through TNFRp55 has a protective role in arthritis induced by oral Yersinia infection.