Detection of intestinal microbiota components in the synovial microenvironment of peripheral spondyloarhritis and its association to immunopathogenic mechanisms

JOSÉ LUIS ARIAS ; EDUARDO CALLEGARI ; DANIELA PAEZ; BLAS RODRIGO; ALICIA MUNARRIZ; RODOLFO PARDO-HIDALGO; HÉCTOR TAMASHIRO; MARÍA SILVIA DI GENARO

Congreso; LXIX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2021

A role for intestinal microbes in arthritis is being considered. We analyzed intra-articularmicrobiota components and their association with immunopathogenic mechanisms ofSpondyloarthritis (SpA). Synovial fluid (SF) of peripheral SpA patients were pooled(n=9): 6 psoriatic arthritis, 2 reactive arthritis, 1 undifferentiated SpA (Protocol approvedCE002-2017). IL-17, IL-6, IL-23 and TGF- levels were quantified in each SF sampleby ELISA and compared with SF from osteoarthritis (OA). Moreover, SW982 cells(human synovial fibroblasts) were incubated with the SpA SF pool in absence or presenceof polymyxin B (LPS inhibitor) and 48 h later the IL-6 levels in the supernatant weremeasured by ELISA. Furthermore, intestinal microbiota proteins were analyzed in theSpA SF pool through a Gel-LC bottom-up mass spectrometry-based proteomic study.Higher levels of IL-17, IL-6, IL-23 and TGF- were detected in SpA compared with OASF (p