Aging modifies the circadian rhythms of Sirt1 and DNA repair enzymes expression in the rat cerebellum. Effect of caloric restriction

CASTRO PASCUAL, IVANNA CARLA; NEVES OLIVEIRA, ANGELA DAS; HELVOORT LENGERT, ANDRE VAN; MELENDEZ, MATÍAS; CARGNELUTTI, ETHELINA; ALTAMIRANO, FERNANDO GABRIEL; FERRAMOLA, MARIANA LUCILA; LACOSTE, MARÍA GABRIELA; DELGADO, SILVIA MARCELA; ANZULOVICH, ANA CECILIA

Virtual

Congreso; XXXVI Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; 2021

Caloric restriction (CR) attenuates the aging process. The circadian molecular machinery and metabolism communicate through Sirtuins. SIRT1 plays a vital role in maintaining genomic integrity, through regulation of the BER repair pathway. We investigated whether in the Sirt1, Ogg1 and Ape1 expression have a temporal pattern in the rat cerebellum, the consequences of aging and the effect of a calorie restricted diet. Male Holtzman: young (3-mo-old), old (22-mo-old) rats fed ad-libitum; and old-CR (22-mo-old rats fed at 40% calorie restricted diet for the last three months) were used. Sirt1 expression showed a circadian oscillation, peaking at night in the cerebellum of the young, aging phase advanced the rhythm of Sirt1 (CT16:07±00:37 vs CT01:31±00:07, p