Immunohistochemical characterization of immune cell subsets on lymph nodes from water buffaloes.

CANTÓN, G.; CHIANINI, F.; KONRAD, J.L.; CAMPERO, C.M.

Uppsala

Congreso; Cutting Edge Pathology 2011; 2011

European Society of Veterinary Pathology (ESVP), European College of Veterinary Pathologists (ECVP), and European Society of Toxicologic Pathology (ESTP)

Introduction: Water buffaloes (Bubalus bubalis) play a crucial role in Asian agriculture and their importance is increasing in western nations. They are susceptible to similar aetiological agents of disease as cattle but the outcome may be different. This may be due to differences in the responses of the immune systems in the two different species. The aim of this work was to characterise immune system cell subsets on fixed lymph nodes from buffaloes. Materials and Methods: Immunohistochemistry was performed on zinc salts fixed paraffin-wax embedded lymph nodes from healthy water buffalo. Monoclonal antibodies (mAbs) were selected from those used in other species or reported previously for water buffalo tissues using other techniques. Results: Specific labelling was observed using mAbs previously unreported in buffalo tissues [EBM11 (macrophages), CC58 (CD8 T-cells), IL-A29 (γσTCR), NKp46 (NK-cells) and HM57 (B cells)] or using clones previously described for use in flow cytometry [MMIA (CD3 T cells), IL-A11 and CC30 (CD4 T-cells)]. Discussion: The results from this study provide a new panel of mAbs to investigate the buffalo inflammatory response to diseases in fixed tissues. Other mAbs previously used in ruminants could also be examined to provide further tools for use in water buffalo tissues