Anti-Mullerian hormone levels in different bovine species and the relationship with invitro embryo production

BERDUGO, J.; TARAZONA-MORALES, A.; ECHEVERRY-ZULUAGA, J.; CARDONA MAYA, W.; LÓPEZ-HERRERA, A.; CRUDELI, G.; KONRAD, J.; ESPASANDIN, G.

REPRODUCTION FERTILITY AND DEVELOPMENT

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2019 vol. 32

1031-3613

The development of markers for reproductive function has been a goal for the embryo production industry and reproductive biology as a method to improve success and increase the ability to explain reproductive phenomena. Cattle and buffalo are bovines that show different reproductive parameters raised in the same conditions. The aim of this work was to compare the results of the in vitro production of cows (Bos indicus) and buffalo (Bubalus bubalis) from animals kept under the same conditions of feeding and handling. This study was conducted in an Argentinian commercial herd of buffalo and cattle located in the province of Corrientes (−27.742859 latitude, −57.773611 longitude) during the breeding season of 2018 (March-May). Twenty animals of each species were used. Anti-Mullerian hormone (AMH) levels of each animal were determined using ELISA. Standardized protocols were used for oocyte aspiration, maturation, fertilisation, and culture of the embryos, and frozen semen of a single proven bull was used in each species. The numbers of follicles, oocytes, and embryos were recorded and analysed individually and grouped by species (Table 1). The normality of the data was evaluated with the D?Agostino and Shapiro-Wilk tests and the comparisons between species using the Mann-Whitney and ANOVA tests, and correlation analysis was performed using the Spearman test. Values are shown as median and range. A P-value