INVESTIGADORES
FARIÑA julia ines
artículos
Título:
Low-cost method for the preservation of Sclerotium rolfsii Proimi F-6656: Inoculum standardization and its use in scleroglucan production
Autor/es:
J.I. FARIÑA; F. SIÑERIZ; O.E. MOLINA; N.I. PEROTTI
Revista:
BIOTECHNOLOGY TECHNIQUES
Editorial:
Springer
Referencias:
Lugar: Dordrecht; Año: 1996 vol. 10 p. 705 - 708
ISSN:
0951-208X
Resumen:
The Castellani´s Method for the preservation of Sclerotium rolfsii in sterile distilled water was tested. Culturing on Potato Dextrosa Yeast extract (PDY) slants, the current system used, was also evaluated. Preservation of sclerotia according to the Castellani´s method allowed the strain survival for more than two years. Comparing with the strain periodically activated, a critical decrease (about 80%) in beta-glucan synthesizing capacity was detected for mycelium preserved either on PDY slants or in water. Activation of stored sclerotia followed by subculturing in liquid Production Medium(PM) allowed preparation of homogeneous suspensions for batch fermentations, and scleroglucanconcentrations achieved were similar to those with the strain periodically activated.
