Protective effect of the flavonoid quercetin on the inhibition of plasma membrane calcium pump by aluminum

ANA SOL RIESCO, MARILINA DE SAUTU, JUAN PABLO ROSSI, MARIELA S FERREIRA-GOMES AND IRENE C MANGIALAVORI; .ROSSI, JUAN PABLO F.C.

LA PLATA

Congreso; XLVII Reunión Anual de la Sociedad de Biofísica Argentina. La Plata; 2018

SOCIEDAD ARGENTINA DE BIOFÍSICA

 Protectiveeffect of the flavonoid quercetin on the inhibition of plasma membrane calciumpump by aluminumAna Sol Riesco, Marilina deSautu, Juan Pablo Rossi,  Mariela SFerreira-Gomes and Irene C Mangialavori.Cátedra de FisicoquímicaBiológica-Departamento de Química Biológica, Universidad de Buenos Aires,Instituto de Química y Fisicoquímica Biológicas (IQUIFIB) UBA-CONICET. Junín 956,Buenos Aires, Argentina. Email: anita_riesco12@hotmail.com Aluminum is etiologically and epidemiologicallyrelated to several neurological disorders, including Alzheimer's, Parkinson'sand amyotrophic lateral sclerosis. Exposure to high levels of this metal leadsto neurofibrillary and neuronal degeneration and induces neurobehavioraldeficits and morphological changes in the brain. The mechanism of aluminumneurotoxicity has been associated with several cellular phenomena, among them,a disturbance of intracellular Ca2+ homeostasis. Effectively, wehave recently demonstrate that Al3+ inhibits the Ca2+-ATPaseactivity of the plasma membrane (PMCA) and sarcoplasmic reticulum (SERCA)calcium pumps [1] and produces an increase in intracellular Ca2+ [2].Flavonoids are commonly found in fruits and vegetables and they are also partof human diet It is known that flavonoids have anti-oxidant activity. They preventmetal-mediated generation of free radicals because they are capable ofsequestering metal ions by chelating In this context, the aim of this work was toevaluate if the flavonoid quercetin protects PMCA from inhibition by aluminumand to characterize the mechanism by which it does so. Our results suggest thatquercetin protects PMCA from aluminum inhibition because it has a chelating effecton this trivalent cation and the quercetin-Al3+ complex does notbind to PMCA. In addition, the presence of quercetin partially reverses theeffect of the inhibitory effect of aluminum, suggesting that its administrationis a potential strategy to reverse the effect of aluminum at the cellularlevel. [1] De Sautu M, Saffioti NA,Ferreira-Gomes MS, Rossi RC, Rossi JPFC and Mangialavori IC. BBA-Biomembrane (2018) 860(8):1580-1588.[2] XLII Congresoda Sociedade Brasileira de Biofisica. Sao Pablo, Brasil. 27-29 de octubre de 2017. ?Inhibitionof the plasma membrane calcium pump by aluminium in vitro and in living cells? De Sautu, M., Ferreira-Gomes M, SaffiotiN, Rinaldi D, Rossi, R, Rossi JPFC and Mangialavori, IC.  This work was supported by ANCYT PICT 2014 0065,CONICET PIP 0250 and Universidad de Buenos Aires 2014-2017: 20020130100254B