INVESTIGADORES
ROSSI juan pablo Francisco
artículos
Título:
Trypsin activation of the red cell Ca2+-pump ATPase is calcium sensitive
Autor/es:
ROSSI,JUAN PABLO; SCHATZMANN, HANS
Revista:
CELL CALCIUM.
Editorial:
CHURCHILL LIVINGSTONE
Referencias:
Lugar: ESCOCIA; Año: 1982 vol. 6 p. 13 - 26
ISSN:
0143-4160
Resumen:
Stimulation of the calmodulin-independent activity of the red cell Ca2+-pump ATPase by trypsin treatment (of calmodulin free red cell membranes) is sensitive to Ca2+ in a concentration range near the KCa of the transport site. The Ca2+ requirement for this effect is absolute, whereas the calmodulin sensitivity of the ATPase can be abolished by sufficient trypsin attack in the absence of Ca2+, although Ca2+ accelerates inactivation. This indicates that the two effects of trypsin are due to at least two distinct cleavage sites in the pump protein.
